Other
Part:BBa_K277072:Design
Designed by: James DiCarlo Group: iGEM09_Johns_Hopkins-BAG (2009-10-21)
3L.3_23.C2.02
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 94
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 181
Illegal SpeI site found at 94 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 94
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 94
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constructed using overlap extension PCR protocol described in RFC38
Source
Synthetic Yeast